PMID:18765794
| Citation |
Pesavento, C, Becker, G, Sommerfeldt, N, Possling, A, Tschowri, N, Mehlis, A and Hengge, R (2008) Inverse regulatory coordination of motility and curli-mediated adhesion in Escherichia coli. Genes Dev. 22:2434-46 |
|---|---|
| Abstract |
During the transition from post-exponential to stationary phase, Escherichia coli changes from the motile-planktonic to the adhesive-sedentary "lifestyle." We demonstrate this transition to be controlled by mutual inhibition of the FlhDC/motility and sigma(S)/adhesion control cascades at two distinct hierarchical levels. At the top level, motility gene expression and the general stress response are inversely coordinated by sigma(70)/sigma(FliA)/sigma(S) competition for core RNA polymerase and the FlhDC-controlled FliZ protein acting as a sigma(S) inhibitor. At a lower level, the signaling molecule bis-(3'-5')-cyclic-diguanosine monophosphate (c-di-GMP) reduces flagellar activity and stimulates transcription of csgD, which encodes an essential activator of adhesive curli fimbriae expression. This c-di-GMP is antagonistically controlled by sigma(S)-regulated GGDEF proteins (mainly YegE) and YhjH, an EAL protein and c-di-GMP phosphodiesterase under FlhDC/FliA control. The switch from motility-based foraging to the general stress response and curli expression requires sigma(S)-modulated down-regulation of expression of the flagellar regulatory cascade as well as proteolysis of the flagellar master regulator FlhDC. Control of YhjH by FlhDC and of YegE by sigma(S) produces a fine-tuned checkpoint system that "unlocks" curli expression only after down-regulation of flagellar gene expression. In summary, these data reveal the logic and sequence of molecular events underlying the motile-to-adhesive "lifestyle" switch in E. coli. |
| Links |
PubMed PMC2532929 Online version:10.1101/gad.475808 |
| Keywords |
Cyclic GMP/analogs & derivatives; Cyclic GMP/physiology; Escherichia coli K12/physiology; Escherichia coli Proteins/metabolism; Fimbriae, Bacterial/physiology; Flagella/physiology; Gene Expression Regulation, Bacterial; Phosphoric Diester Hydrolases/metabolism; Trans-Activators/metabolism |
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