PMID:1860830
| Citation |
Petersen, SK and Hansen, FG (1991) A missense mutation in the rpoC gene affects chromosomal replication control in Escherichia coli. J. Bacteriol. 173:5200-6 |
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| Abstract |
An RNA polymerase mutant with a single-base-pair change in the rpoC gene affects chromosome initiation control. The mutation, which is recessive, is a G to A transition leading to the substitution of aspartate for glycine at amino acid residue 1033 in the RNA polymerase beta' subunit. The chromosome copy number is increased twofold in the mutant at semipermissive growth temperatures (39 degrees C). In a delta oriC strain, in which chromosome initiation is governed by an F replicon, chromosome copy number is not affected. Plasmid pBR322 copy number is also increased in the mutant at 39 degrees C. The mutation causes a more than fivefold increased expression of the dnaA gene at 39 degrees C. It is conceivable that it is this high DnaA concentration which causes the high chromosome copy number and that the mutant RNA polymerase beta' subunit exerts its effect by altering the expression of the dnaA gene. However, other factors must be affected as well to explain why the RNA polymerase mutant can grow in a balanced fashion with a high chromosome concentration. This is in contrast to wild-type cells, which exhibit higher origin concentrations when DnaA protein is overproduced, but in which the overall DNA concentration is only moderately affected. |
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| Keywords |
Aspartic Acid/genetics; Bacterial Proteins/genetics; Chromosomes, Bacterial/metabolism; DNA Replication/genetics; DNA-Binding Proteins; DNA-Directed RNA Polymerases/genetics; Escherichia coli/genetics; Gene Expression Regulation, Bacterial/genetics; Genetic Complementation Test; Glycine/genetics; Immunoblotting; Kinetics; Mutation/genetics; Phenotype; Temperature |
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