PMID:1853567
| Citation |
Bamford, JK, Hänninen, AL, Pakula, TM, Ojala, PM, Kalkkinen, N, Frilander, M and Bamford, DH (1991) Genome organization of membrane-containing bacteriophage PRD1. Virology 183:658-76 |
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| Abstract |
We have determined the nucleotide sequence of the late region (11 kbp) of the lipid-containing bacteriophage PRD1. Gene localization was carried out by complementing nonsense phage mutants with genomic clones containing specific reading frames. The localization was confirmed by sequencing the N-termini of isolated gene products as well as sequencing the N-termini of tryptic fragments of the phage membrane-associated proteins. This, with the previously obtained sequence of the early regions, allowed us to organize most of the phage genes in the phage genome. |
| Links | |
| Keywords |
Amino Acid Sequence; Bacteriophages/genetics; Base Sequence; Cloning, Molecular; DNA, Viral; Electrophoresis, Polyacrylamide Gel; Genes, Viral; Genetic Complementation Test; Gram-Negative Bacteria; Molecular Sequence Data; Mutation; Open Reading Frames; Restriction Mapping; Viral Envelope Proteins/genetics |
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