PMID:1851291
| Citation |
Reece, RJ and Maxwell, A (1991) The C-terminal domain of the Escherichia coli DNA gyrase A subunit is a DNA-binding protein. Nucleic Acids Res. 19:1399-405 |
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| Abstract |
We have constructed a clone which over-produces a 33 kDa protein representing the C-terminal portion of the Escherichia coli DNA gyrase A subunit. This protein has no enzymic activity of its own, but will form a complex with a 64 kDa protein (representing the N-terminal part of the A subunit) and the gyrase B subunit, that will efficiently catalyse DNA supercoiling. We show that the 33 kDa protein can bind to DNA on its own in a manner which induces positive supercoiling of the DNA. We propose that the 33 kDa protein represents a domain of the gyrase A subunit which is involved in the wrapping of DNA around DNA gyrase. |
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| Keywords |
Base Sequence; Cloning, Molecular; DNA Topoisomerases, Type II/genetics; DNA Topoisomerases, Type II/metabolism; DNA, Bacterial/genetics; DNA, Bacterial/metabolism; DNA, Superhelical/genetics; DNA, Superhelical/metabolism; DNA-Binding Proteins/genetics; DNA-Binding Proteins/metabolism; Electrophoresis, Agar Gel; Escherichia coli/enzymology; Molecular Sequence Data |
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