PMID:1849507
| Citation |
Trimbur, DE and Mortlock, RP (1991) Isolation and characterization of Escherichia coli mutants able to utilize the novel pentose L-ribose. J. Bacteriol. 173:2459-64 |
|---|---|
| Abstract |
Wild-type strains of Escherichia coli were unable to utilize L-ribose for growth. However, L-ribose-positive mutants could be isolated from strains of E. coli K-12 which contained a ribitol operon. L-ribose-positive strains of E. coli, isolated after 15 to 20 days, had a growth rate of 0.22 generation per h on L-ribose. Growth on L-ribose was found to induce the enzymes of the L-arabinose and ribitol pathways, but only ribitol-negative mutants derived from strains originally L-ribose positive lost the ability to grow on L-ribose, showing that a functional ribitol pathway was required. One of the mutations permitting growth on L-ribose enabled the mutants to produce constitutively an NADPH-linked reductase which converted L-ribose to ribitol. L-ribose is not metabolized by an isomerization to L-ribulose, as would be predicted on the basis of other pentose pathways in enteric bacteria. Instead, L-ribose was metabolized by the reduction of L-ribose to ribitol, followed by the conversion to D-ribulose by enzymes of the ribitol pathway. |
| Links | |
| Keywords |
Aldose-Ketose Isomerases; Arabinose/metabolism; Carbohydrate Epimerases/analysis; Chromatography, Thin Layer; Chromosome Mapping; Electrophoresis; Escherichia coli/enzymology; Escherichia coli/genetics; Escherichia coli/metabolism; Mutation; Phosphotransferases/analysis; Phosphotransferases (Alcohol Group Acceptor); Ribose/metabolism; Sugar Alcohol Dehydrogenases/analysis; Transduction, Genetic |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.