PMID:1848300
| Citation |
Schultz, JE and Matin, A (1991) Molecular and functional characterization of a carbon starvation gene of Escherichia coli. J. Mol. Biol. 218:129-40 |
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| Abstract |
Escherichia coli induces the synthesis of at least 30 proteins at the onset of carbon starvation, two-thirds of which are positively regulated by the cyclic AMP (cAMP) and cAMP receptor protein (CRP) complex. Two of the cAMP-CRP-dependent genes mapped to 14 and 93 minutes of the chromosome and are designated cstA and cstB, respectively. The cstA promoter region was cloned and localized to a 600 base-pair fragment downstream from the iron-regulated entCEBA-P15 operon. Carbon starvation-inducible transcription initiated at three sites spaced one turn of the DNA helix apart. All had--10 sequences similar to consensus E sigma 70 promoters and poor--35 sequences. Deletion of a putative CRP binding site abolished carbon starvation-mediated induction. Sequence analysis of the cstA coding region revealed the presence of three sequential open reading frames potentially encoding two hydrophobic proteins of 60,223 Da and 15,201 Da and a hydrophilic protein of 7467 Da. Overexpression of the cstA region produced starvation-inducible proteins of the expected sizes. Suggestive evidence was obtained that cstA is involved in peptide utilization. |
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| Keywords |
Amino Acid Sequence; Bacterial Proteins/biosynthesis; Bacterial Proteins/genetics; Bacterial Proteins/isolation & purification; Base Sequence; Carbon/metabolism; Chromosomes, Bacterial; Cyclic AMP Receptor Protein/metabolism; DNA, Bacterial/genetics; Escherichia coli/genetics; Escherichia coli/growth & development; Escherichia coli/metabolism; Genes, Bacterial; Genotype; Molecular Sequence Data; Open Reading Frames; Plasmids; Protein Conformation; Restriction Mapping; Transcription, Genetic; Transduction, Genetic |
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