PMID:18482579
| Citation |
Mihara, H, Hidese, R, Yamane, M, Kurihara, T and Esaki, N (2008) The iscS gene deficiency affects the expression of pyrimidine metabolism genes. Biochem. Biophys. Res. Commun. 372:407-11 |
|---|---|
| Abstract |
Inactivation of iscS encoding cysteine desulfurase results in a slow growth phenotype associated with the deficiency of iron-sulfur clusters, thiamine, NAD, and tRNA thionucleosides in Escherichia coli. However, the other roles of iscSin vivo are unknown. By using differential screening strategies, we identified 2 pyrimidine salvage enzymes, namely, uridine phosphorylase and cytidine deaminase, which were down-regulated in the iscS mutant. Both enzymes are positively regulated by the cAMP receptor protein (CRP). We also identified a novel protein complex, namely, YeiT-YeiA, whose expression level was decreased in the iscS mutant. The recombinant YeiT-YeiA complex exhibited NADH-dependent dihydropyrimidine dehydrogenase activity, indicating its role in pyrimidine metabolism. The presence of a CRP-binding consensus sequence on the 5'-upstream of the yeiT-YeiA gene suggests its regulation by CRP. These results provide a clue to the possible role of iscS in pyrimidine metabolism by gene regulation. |
| Links |
PubMed Online version:10.1016/j.bbrc.2008.05.019 |
| Keywords |
Base Sequence; Carbon-Sulfur Lyases/genetics; Carbon-Sulfur Lyases/physiology; Consensus Sequence; Cyclic AMP Receptor Protein/metabolism; Cytidine Deaminase/genetics; Cytidine Deaminase/metabolism; Dihydrouracil Dehydrogenase (NAD+)/genetics; Dihydrouracil Dehydrogenase (NAD+)/metabolism; Dihydrouracil Dehydrogenase (NADP)/metabolism; Down-Regulation; Electrophoresis, Gel, Two-Dimensional; Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli Proteins/genetics; Escherichia coli Proteins/metabolism; Escherichia coli Proteins/physiology; Gene Expression Regulation, Bacterial; Mutation; Pyrimidines/metabolism; Transcription Factors/metabolism; Uridine Phosphorylase/genetics; Uridine Phosphorylase/metabolism |
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