PMID:1846850
| Citation |
Ikeda, M, Wachi, M, Jung, HK, Ishino, F and Matsuhashi, M (1991) The Escherichia coli mraY gene encoding UDP-N-acetylmuramoyl-pentapeptide: undecaprenyl-phosphate phospho-N-acetylmuramoyl-pentapeptide transferase. J. Bacteriol. 173:1021-6 |
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| Abstract |
Amplification of the mraY gene, previously called open reading frame Y (ORF-Y, 1,080 bp), at 2 min in the chromosome map of Escherichia coli enhanced the activity of UDP-N-acetylmuramoyl-pentapeptide: undecaprenyl-phosphate phospho-N-acetylmuramoyl-pentapeptide transferase (EC 2.7.8.13). This enzyme catalyzes the formation of undecaprenyl-pyrophosphoryl-N-acetylmuramoyl-pentapeptide from UDP-N-acetylmuramoyl-pentapeptide and undecaprenyl-phosphate, the first step in the lipid cycle reactions in biosynthesis of bacterial cell wall peptidoglycans. The enhanced enzyme activity was sensitive to tunicamycin, and the amino tunicamycin-sensitive N-acetylglucosamine-1-phosphate transferase of Saccharomyces cerevisiae. Very probably mraY is the structural gene for the above enzyme. |
| Links | |
| Keywords |
Amino Acid Sequence; Escherichia coli/enzymology; Escherichia coli/genetics; Genes, Bacterial; Kinetics; Molecular Sequence Data; Phosphotransferases/chemistry; Phosphotransferases/genetics; Phosphotransferases/metabolism; Plasmids; Restriction Mapping; Saccharomyces cerevisiae/enzymology; Saccharomyces cerevisiae/genetics; Sequence Homology, Nucleic Acid; Transferases (Other Substituted Phosphate Groups); Tunicamycin/pharmacology; Uridine Diphosphate N-Acetylmuramic Acid/analogs & derivatives; Uridine Diphosphate N-Acetylmuramic Acid/metabolism; Uridine Monophosphate/metabolism |
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