PMID:18433631

Citation	Efromovich, S, Grainger, D, Bodenmiller, D and Spiro, S (2008) Genome-wide identification of binding sites for the nitric oxide-sensitive transcriptional regulator NsrR. Meth. Enzymol. 437:211-33
Abstract	NsrR is a nitric oxide-sensitive regulator of transcription. In Escherichia coli, NsrR is a repressor of the hmp gene encoding the flavohemoglobin that detoxifies nitric oxide. Three other transcription units (ytfE, ygbA, and hcp-hcr) are known to be subject to regulation by NsrR. This chapter describes experimental and statistical protocols used to identify NsrR-binding sites in the E. coli chromosome using chromatin immunoprecipitation and microarray analysis. The methods are applicable, with suitable modifications, to any regulatory protein and any organism.
Links	PubMed Online version:10.1016/S0076-6879(07)37012-2
Keywords	Automatic Data Processing/methods; Binding Sites; Chromatin Immunoprecipitation/methods; Chromosome Mapping; Cloning, Organism/methods; DNA-Binding Proteins/metabolism; DNA-Binding Proteins/physiology; Escherichia coli Proteins/metabolism; Escherichia coli Proteins/physiology; Gene Expression Profiling/methods; Gene Expression Regulation, Bacterial/drug effects; Genome, Bacterial; Microbiological Techniques; Models, Biological; Nitric Oxide/pharmacology; Oligonucleotide Array Sequence Analysis/methods; Protein Binding; Staining and Labeling/methods; Transcription Factors/metabolism; Transcription Factors/physiology
edit table

Significance

You can help EcoliWiki by summarizing why this paper is useful

You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.

Add links to pages that link here (e.g. gene, product, method pages)

See Help:References for how to manage references in EcoliWiki.