PMID:18421347
| Citation |
Isalan, M, Lemerle, C, Michalodimitrakis, K, Horn, C, Beltrao, P, Raineri, E, Garriga-Canut, M and Serrano, L (2008) Evolvability and hierarchy in rewired bacterial gene networks. Nature 452:840-5 |
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| Abstract |
Sequencing DNA from several organisms has revealed that duplication and drift of existing genes have primarily moulded the contents of a given genome. Though the effect of knocking out or overexpressing a particular gene has been studied in many organisms, no study has systematically explored the effect of adding new links in a biological network. To explore network evolvability, we constructed 598 recombinations of promoters (including regulatory regions) with different transcription or sigma-factor genes in Escherichia coli, added over a wild-type genetic background. Here we show that approximately 95% of new networks are tolerated by the bacteria, that very few alter growth, and that expression level correlates with factor position in the wild-type network hierarchy. Most importantly, we find that certain networks consistently survive over the wild type under various selection pressures. Therefore new links in the network are rarely a barrier for evolution and can even confer a fitness advantage. |
| Links |
PubMed PMC2666274 Online version:10.1038/nature06847 |
| Keywords |
Escherichia coli/genetics; Escherichia coli/growth & development; Escherichia coli/metabolism; Escherichia coli Proteins/genetics; Escherichia coli Proteins/metabolism; Evolution, Molecular; Gene Expression Regulation, Bacterial/genetics; Gene Regulatory Networks/genetics; Genes, Bacterial/genetics; Genetic Engineering; Heat-Shock Response; Oligonucleotide Array Sequence Analysis; Open Reading Frames/genetics; Promoter Regions, Genetic/genetics; Selection, Genetic; Serial Passage; Sigma Factor/genetics; Sigma Factor/metabolism; Transcription Factors/genetics; Transcription Factors/metabolism |
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