PMID:18419149
| Citation |
Lok, CN, Ho, CM, Chen, R, Tam, PK, Chiu, JF and Che, CM (2008) Proteomic identification of the Cus system as a major determinant of constitutive Escherichia coli silver resistance of chromosomal origin. J. Proteome Res. 7:2351-6 |
|---|---|
| Abstract |
Although silver is one of the most potent and rapidly acting toxic metals to bacteria, silver-resistant bacteria do exist with low incidence. A proteomic approach was employed to identify the silver resistance determinants of a silver-resistant Escherichia coli strain isolated from stepwise selection against increasing concentrations of silver (Li et al. J. Bacteriol 1997, 179, 6127-32). Two-dimensional gel electrophoresis and mass spectrometry analysis revealed that members of the CusCFBA copper/silver chemiosmotic efflux system were highly expressed in the silver-resistant strain but undetectable in the parental silver-sensitive strain. Disruption of the cus locus of the silver-resistant strain resulted in a decrease of the minimum inhibitory concentration of Ag (+) from more than 1 mM to 12 microM. These results suggest that the chromosomally encoded Cus system, which naturally controls the periplasmic copper concentrations, is selectable to confer a constitutive silver resistance phenotype. |
| Links |
PubMed Online version:10.1021/pr700646b |
| Keywords |
Bacterial Outer Membrane Proteins/analysis; Bacterial Outer Membrane Proteins/metabolism; Cation Transport Proteins/analysis; Cation Transport Proteins/genetics; Cation Transport Proteins/metabolism; Copper Sulfate/pharmacology; Drug Resistance, Bacterial; Electrophoresis, Gel, Two-Dimensional; Escherichia coli/drug effects; Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli Proteins/analysis; Escherichia coli Proteins/genetics; Escherichia coli Proteins/metabolism; Gene Deletion; Mutation; Proteomics/methods; Silver/pharmacology; Silver Nitrate/pharmacology; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Tandem Mass Spectrometry |
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