PMID:1834669
| Citation |
Berg, BL, Li, J, Heider, J and Stewart, V (1991) Nitrate-inducible formate dehydrogenase in Escherichia coli K-12. I. Nucleotide sequence of the fdnGHI operon and evidence that opal (UGA) encodes selenocysteine. J. Biol. Chem. 266:22380-5 |
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| Abstract |
The fdnGHI operon of Escherichia coli encodes nitrate-inducible formate dehydrogenase. We report here the entire nucleotide sequence of fdnGHI. The sequence contains three open reading frames of sizes appropriate to encode the three subunits of formate dehydrogenase-N. fdnG contains an in-frame UGA codon that specifies selenocysteine incorporation, and the predicted amino acid sequence of FdnG shows similarity to two other bacterial formate dehydrogenase enzymes. FdnH contains 4 cysteine clusters typical of those found in iron-sulfur proteins. FdnG also contains a cysteine cluster. Evidence from sequence and spectral analyses suggest that FdnI encodes cytochrome bFdn556. Implications for the membrane topology of formate dehydrogenase-N and its mechanism of proton translocation are discussed. |
| Links | |
| Keywords |
Amino Acid Sequence; Base Sequence; Cloning, Molecular; Codon/genetics; Cysteine/analogs & derivatives; Cysteine/metabolism; Cytochrome b Group/genetics; Enzyme Induction; Escherichia coli/drug effects; Escherichia coli/enzymology; Escherichia coli/genetics; Formate Dehydrogenases/biosynthesis; Formate Dehydrogenases/genetics; Gene Expression Regulation, Bacterial/drug effects; Gene Expression Regulation, Enzymologic/drug effects; Genes, Bacterial/drug effects; Genotype; Molecular Sequence Data; Nitrates/pharmacology; Oligodeoxyribonucleotides; Open Reading Frames; Operon; Organoselenium Compounds/metabolism; Plasmids; Recombinant Fusion Proteins/metabolism; Restriction Mapping; Selenocysteine; Sequence Homology, Nucleic Acid; beta-Galactosidase/genetics; beta-Galactosidase/metabolism |
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