PMID:1833388
| Citation |
Gross, G (1991) RNase E cleavage in the atpE leader region of atpE/interferon-beta hybrid transcripts in Escherichia coli causes enhanced rates of mRNA decay. J. Biol. Chem. 266:17880-4 |
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| Abstract |
Chimeric transcripts containing the ribosome binding site of the Escherichia coli atpE gene and variants of the human structural interferon-beta gene are subject to RNase E processing in the 5'-untranslated atpE part of the transcripts. The absence of processing at two sites in the atpE leader-sequence caused by the RNase E deficiency in E. coli host N3431 leads to a considerable stabilization of the mRNA moiety. RNase E has originally been described as a processing enzyme for non-mRNAs such as precursor 5 S rRNA and RNA1, but cleavage mRNA substrates have also been reported. RNase E processing of the atpE gene leader sequence-containing transcripts leads to an increased rate of mRNA breakdown. The two RNase E-dependent processing sites in the atpE part of the mRNA transcripts exhibit some similarity to the other known RNase E processing sites. The influence of RNase E cleavage upon post-transcriptional regulation such as RNA stability and the efficiency of translational initiation is discussed. |
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| Keywords |
Bacteriophage lambda/genetics; Base Sequence; Endoribonucleases/metabolism; Escherichia coli/genetics; Genes, Bacterial; Genes, Viral; Hydrolysis; Interferon Type I/genetics; Kinetics; Molecular Sequence Data; Promoter Regions, Genetic; Protein Biosynthesis; Protein Sorting Signals/genetics; RNA, Messenger/metabolism; Substrate Specificity; Transcription, Genetic |
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