PMID:18310334
| Citation |
Kolodkin-Gal, I and Engelberg-Kulka, H (2008) The extracellular death factor: physiological and genetic factors influencing its production and response in Escherichia coli. J. Bacteriol. 190:3169-75 |
|---|---|
| Abstract |
Gene pairs specific for a toxin and its antitoxin are called toxin-antitoxin modules and are found on the chromosomes of many bacteria. The most studied of these modules is Escherichia coli mazEF, in which mazF encodes a stable toxin, MazF, and mazE encodes a labile antitoxin, MazE, which prevents the lethal effect of MazF. In a previous report from this laboratory, it was shown that mazEF-mediated cell death is a population phenomenon requiring a quorum-sensing peptide called the extracellular death factor (EDF). EDF is the linear pentapeptide NNWNN (32). Here, we further confirm that EDF is a signal molecule in a mixed population. In addition, we characterize some physiological conditions and genes required for EDF production and response. Furthermore, stress response and the gene specifying MazEF, the Zwf (glucose-6-phosphate dehydrogenase) gene, and the protease ClpXP are critical in EDF production. Significant strain differences in EDF production and response explain variations in the induction of mazEF-mediated cell death. |
| Links |
PubMed PMC2347391 Online version:10.1128/JB.01918-07 |
| Keywords |
Apoptosis; DNA-Binding Proteins/genetics; DNA-Binding Proteins/metabolism; Endopeptidase Clp/genetics; Endopeptidase Clp/metabolism; Endoribonucleases/genetics; Endoribonucleases/metabolism; Escherichia coli/genetics; Escherichia coli/physiology; Escherichia coli Proteins/genetics; Escherichia coli Proteins/metabolism; Glucosephosphate Dehydrogenase/genetics; Glucosephosphate Dehydrogenase/metabolism; Oligopeptides/biosynthesis; Oligopeptides/genetics; Quorum Sensing |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
See also
References
See Help:References for how to manage references in EcoliWiki.