PMID:18288206
| Citation |
Das, G, Thotala, DK, Kapoor, S, Karunanithi, S, Thakur, SS, Singh, NS and Varshney, U (2008) Role of 16S ribosomal RNA methylations in translation initiation in Escherichia coli. EMBO J. 27:840-51 |
|---|---|
| Abstract |
Translation initiation from the ribosomal P-site is the specialty of the initiator tRNAs (tRNA(fMet)). Presence of the three consecutive G-C base pairs (G29-C41, G30-C40 and G31-C39) in their anticodon stems, a highly conserved feature of the initiator tRNAs across the three kingdoms of life, has been implicated in their preferential binding to the P-site. How this feature is exploited by ribosomes has remained unclear. Using a genetic screen, we have isolated an Escherichia coli strain, carrying a G122D mutation in folD, which allows initiation with the tRNA(fMet) containing mutations in one, two or all the three G-C base pairs. The strain shows a severe deficiency of methionine and S-adenosylmethionine, and lacks nucleoside methylations in rRNA. Targeted mutations in the methyltransferase genes have revealed a connection between the rRNA modifications and the fundamental process of the initiator tRNA selection by the ribosome. |
| Links |
PubMed PMC2274930 Online version:10.1038/emboj.2008.20 |
| Keywords |
5,10-Methylenetetrahydrofolate Reductase (FADH2)/biosynthesis; 5,10-Methylenetetrahydrofolate Reductase (FADH2)/genetics; Amino Acid Substitution/genetics; Base Sequence; Escherichia coli Proteins/biosynthesis; Escherichia coli Proteins/genetics; Methylation; Molecular Sequence Data; Mutation; Peptide Chain Initiation, Translational/genetics; RNA, Bacterial/metabolism; RNA, Ribosomal, 16S/metabolism; RNA, Ribosomal, 16S/physiology; RNA, Transfer, Met/genetics; Ribosomes/metabolism |
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