PMID:1827085
| Citation |
Prakash, A, Valinluck, B and Ryu, J (1991) Genomic hsd-Mu(lac) operon fusion mutants of Escherichia coli K-12. Gene 99:9-14 |
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| Abstract |
Genomic (chromosomal)hsd-Mu(lac) operon fusions have been constructed in two strains of Escherichia coli K-12 for the three hsd genes, hsdRK, hsdMK and hsdSK, using MudX and lambda placMu53. Expression of hsdK mutants ranged from 16 to 74 units (u) (with a mean of 52 u) for fusions to promoter pres and ranged from 26-75 u (also with a mean of 52 u) for fusions to promoter pmod. The expression of the two hsdK promoters was measured in different stages of growth. The pres fusion mutant showed a lag in beta-galactosidase (beta Gal) production, as compared to the pmod fusion mutant. One r-Km-K mutant (JR205) showed more than ten times the beta Gal activity of other insertion mutants. The activity of this mutant decreased by 20-fold upon the transfer of F101-102, which includes the wild-type hsd region. Positive gene-dosage effect was observed using F' plasmids containing the hsd-lacZ region. |
| Links | |
| Keywords |
Bacteriophage mu/genetics; Chromosomes, Bacterial; Cloning, Molecular; Escherichia coli/genetics; Escherichia coli/growth & development; F Factor; Genes, Bacterial; Lac Operon; Mutagenesis; Phenotype; Promoter Regions, Genetic; Restriction Mapping |
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