PMID:1826904
| Citation |
Kornitzer, D, Teff, D, Altuvia, S and Oppenheim, AB (1991) Isolation, characterization, and sequence of an Escherichia coli heat shock gene, htpX. J. Bacteriol. 173:2944-53 |
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| Abstract |
We isolated and characterized a new Escherichia coli gene, htpX. The htpX gene has been localized at min 40.3 on the chromosome. We determined its transcription and translation start site. htpX expresses a 32-kDa protein from a monocistronic transcript; expression of this protein is induced by temperature upshift. htpX is expressed from a sigma 32-dependent promoter and is thus part of the heat shock regulon. Cells carrying a htpX gene disruption grow well at all temperatures and under all conditions tested and have no apparent phenotype. However, cells which overexpress a truncated form of the protein display a higher rate of degradation of puromycyl peptides. |
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| Keywords |
Amino Acid Sequence; Bacteriophage lambda; Base Sequence; Blotting, Northern; Blotting, Southern; Blotting, Western; Escherichia coli/genetics; Escherichia coli Proteins; Genes, Bacterial; Heat-Shock Proteins/genetics; Heat-Shock Proteins/physiology; Hot Temperature; Lysogeny/drug effects; Molecular Sequence Data; Plasmids; Promoter Regions, Genetic/genetics; Proteins/metabolism; RNA, Messenger/analysis; Restriction Mapping |
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