PMID:18265058
| Citation |
Bonifacino, JS (2001) Metabolic labeling with amino acids. Curr Protoc Mol Biol Chapter 10:Unit 10.18 |
|---|---|
| Abstract |
Metabolic labeling techniques are used to study biosynthesis, processing, intracellular transport, secretion, degradation, and physical-chemical properties of proteins. This update focuses on pulse-labeling and pulse-chase experiments done with [(35)S]-methionine, but gives directions for labeling with other radiolabeled amino acids, and also offers guidance for the safe use and handling of 35S-labeled compounds. |
| Links |
PubMed Online version:10.1002/0471142727.mb1018s44 |
| Keywords |
Amino Acids; Animals; Cells, Cultured; Chemical Precipitation; Humans; Isotope Labeling/methods; Methionine; Proteins/metabolism; Sulfur Radioisotopes; Time Factors; Trichloroacetic Acid |
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