PMID:18236465
| Citation |
Vandenburgh, H, Shansky, J, Benesch-Lee, F, Barbata, V, Reid, J, Thorrez, L, Valentini, R and Crawford, G (2008) Drug-screening platform based on the contractility of tissue-engineered muscle. Muscle Nerve 37:438-47 |
|---|---|
| Abstract |
A tissue-based approach to in vitro drug screening allows for determination of the cumulative positive and negative effects of a drug at the tissue rather than the cellular or subcellular level. Skeletal muscle myoblasts were tissue-engineered into three-dimensional muscle with parallel myofibers generating directed forces. When grown attached to two flexible micro-posts (mu posts) acting as artificial tendons in a 96-well plate format, the miniature bioartificial muscles (mBAMs) generated tetanic (active) forces upon electrical stimulation measured with a novel image-based motion detection system. mBAM myofiber hypertrophy and active force increased in response to insulin-like growth factor 1. In contrast, mBAM deterioration and weakness was observed with a cholesterol-lowering statin. The results described in this study demonstrate the integration of tissue engineering and biomechanical testing into a single platform for the screening of compounds affecting muscle strength. |
| Links |
PubMed Online version:10.1002/mus.20931 |
| Keywords |
Animals; Biological Assay/instrumentation; Biological Assay/methods; Cells, Cultured; Drug Evaluation, Preclinical/instrumentation; Drug Evaluation, Preclinical/methods; Electric Stimulation; Heptanoic Acids/pharmacology; Hydroxymethylglutaryl-CoA Reductase Inhibitors/pharmacology; Insulin-Like Growth Factor I/pharmacology; Male; Mice; Mice, Inbred C3H; Muscle Contraction/drug effects; Muscular Atrophy/drug therapy; Muscular Atrophy/physiopathology; Myoblasts/cytology; Myoblasts/drug effects; Pyrroles/pharmacology; Tissue Engineering/instrumentation; Tissue Engineering/methods |
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