PMID:18235183
| Citation |
Syutsubo, K, Yoochatchaval, W, Yoshida, H, Nishiyama, K, Okawara, M, Sumino, H, Araki, N, Harada, H and Ohashi, A (2008) Changes of microbial characteristics of retained sludge during low-temperature operation of an EGSB reactor for low-strength wastewater treatment. Water Sci. Technol. 57:277-81 |
|---|---|
| Abstract |
In this study, a lab scale EGSB reactor was operated for 400 days to investigate the influence of temperature-decrease on the microbial characteristic of retained sludge. The EGSB reactor was started-up at 15 degrees C seeding with 20 degrees C-grown granular sludge. The influent COD of synthetic wastewater was set at 0.6-0.8 gCOD/L. The process-temperature was stepwise reduced from 15 degrees C to 5 degrees C during 400 days operation. Decrease of temperature of the reactor from 15 degrees C to 10 degrees C caused the decline of COD removal efficiency. However, continuous operation of the EGSB reactor led the efficient treatment of wastewater (70% of COD removal, 50% of methane recovery) at 10 degrees C. We confirmed that the both acetate-fed and hydrogen-fed methanogenic activities of retained sludge clearly increased under 15 to 20 degrees C. Changes of microbial profiles of methanogenic bacteria were analyzed by 16S rDNA-targeted DGGE analysis and cloning. It shows that genus Methanospirillum as hydrogen-utilizing methanogen proliferated due to low temperature operation of the reactor. On the other hand, genus Methanosaeta presented in abundance as acetoclastic-methanogen throughout the experiment. |
| Links |
PubMed Online version:10.2166/wst.2008.077 |
| Keywords |
Bioreactors; Methanospirillum/genetics; Phylogeny; RNA, Ribosomal/genetics; Sewage/microbiology; Temperature; Waste Disposal, Fluid/methods |
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