PMID:18235043
| Citation |
Sealy, R, Chaka, W, Surman, S, Brown, SA, Cresswell, P and Hurwitz, JL (2008) Target peptide sequence within infectious human immunodeficiency virus type 1 does not ensure envelope-specific T-helper cell reactivation: influences of cysteine protease and gamma interferon-induced thiol reductase activities. Clin. Vaccine Immunol. 15:713-9 |
|---|---|
| Abstract |
Recent clinical trials have shown that the presence of a robust human immunodeficiency virus type 1 (HIV-1)-specific T-cell response may not be sufficient to prevent or control HIV-1 infection. Studies of antigen processing in the context of infectious HIV-1 are therefore warranted. Envelope-specific, major histocompatibility complex class II-restricted murine T-cell hybridomas were tested for responsiveness to splenic antigen-presenting cells exposed to HIV-1-infected GHOST cells. Interleukin-2 assays showed that the presence of a peptide within HIV-1 did not ensure the reactivation of peptide-specific T cells. Further experiments defined the impact of gamma interferon-induced thiol reductase and cysteine proteases on the processing of HIV-1 peptides. The results highlight potential influences of peptide context on T-cell reactivation by HIV-1 and encourage the continued study of antigen processing as support for improved vaccine design. |
| Links |
PubMed PMC2292667 Online version:10.1128/CVI.00412-07 |
| Keywords |
Animals; Antigen Presentation/immunology; Cysteine Endopeptidases/immunology; Cysteine Endopeptidases/metabolism; Erythrocyte Membrane/immunology; HIV Infections/enzymology; HIV Infections/immunology; HIV Infections/virology; HIV-1/immunology; Histocompatibility Antigens Class II/immunology; Humans; Hybridomas; Interferon-gamma/immunology; Interleukin-2; Leupeptins/immunology; Lymphocyte Activation; Mice; Oxidoreductases/immunology; Oxidoreductases/metabolism; T-Lymphocytes, Helper-Inducer/immunology; env Gene Products, Human Immunodeficiency Virus/immunology |
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