PMID:18234878
| Citation |
Goldmeyer, J, Li, H, McCormac, M, Cook, S, Stratton, C, Lemieux, B, Kong, H, Tang, W and Tang, YW (2008) Identification of Staphylococcus aureus and determination of methicillin resistance directly from positive blood cultures by isothermal amplification and a disposable detection device. J. Clin. Microbiol. 46:1534-6 |
|---|---|
| Abstract |
A simple, rapid, and user-friendly procedure has been developed to identify Staphylococcus aureus and determine its methicillin resistance directly from gram-positive cocci in cluster-containing blood culture medium. The specimens were diluted and heated prior to amplification of the nuc and mecA genes with isothermal helicase-dependent amplification. Amplicons were detected using a disposable detection device. The analytical sensitivity of the assays was 50 CFU per reaction, and the clinical sensitivity and specificity were both 100% for S. aureus detection and 100% and 98% for methicillin resistance determination, respectively. |
| Links |
PubMed PMC2292917 Online version:10.1128/JCM.02234-07 |
| Keywords |
Bacterial Proteins/genetics; Blood/microbiology; Culture Media; DNA Helicases/metabolism; Disposable Equipment; Endonucleases/genetics; Humans; Methicillin Resistance; Micrococcal Nuclease/genetics; Nucleic Acid Amplification Techniques/instrumentation; Nucleic Acid Amplification Techniques/methods; Reagent Kits, Diagnostic; Sensitivity and Specificity; Staphylococcus aureus/classification; Staphylococcus aureus/drug effects; Staphylococcus aureus/genetics; Staphylococcus aureus/isolation & purification |
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