PMID:18230416

Citation	Zhang, X, Griko, NB, Corona, SK and Bulla, LA Jr (2008) Enhanced exocytosis of the receptor BT-R(1) induced by the Cry1Ab toxin of Bacillus thuringiensis directly correlates to the execution of cell death. Comp. Biochem. Physiol. B, Biochem. Mol. Biol. 149:581-8
Abstract	Cry1Ab toxin produced by Bacillus thuringiensis exerts insecticidal action upon binding to BT-R(1), a cadherin receptor localized in the midgut epithelium of the tobacco hornworm Manduca sexta. The univalent binding of toxin to receptor transmits a death signal into the cell and turns on a multi-step signal transduction pathway involving adenylyl cyclase (AC) and protein kinase A (PKA), which drives the biochemical events that culminate in oncotic cell death. Here, we report that cell killing by the Cry1Ab toxin is a dynamic episode in which the toxin promotes exocytotic transport of BT-R(1) from intracellular membrane vesicles to the plasma membrane. The resultant dramatic increase in BT-R(1) displayed on the surface of toxin-treated cells effects the recruitment and concomitant binding of additional toxin monomers which, in turn, amplifies the original signal in a cascade-like manner. Blocking the activation of AC/PKA signal transduction by either EDTA or PKAi inhibits exocytotic trafficking of BT-R(1) and prevents cell death. Moreover, the exocytosis inhibitor Exo1 blocks translocation of receptor and progression of cell death alike. Obviously, movement of BT-R(1) is mediated by toxin-induced signal transduction and amplification of this signaling apparently is critical to the execution of cell death.
Links	PubMed Online version:10.1016/j.cbpb.2007.12.006
Keywords	Animals; Bacillus thuringiensis/metabolism; Bacterial Proteins/metabolism; Bacterial Proteins/toxicity; Bacterial Toxins/metabolism; Bacterial Toxins/toxicity; Cadherins/metabolism; Cell Adhesion/drug effects; Cell Death/drug effects; Cell Line; Cell Membrane/metabolism; Cytotoxins/metabolism; Cytotoxins/toxicity; Endocytosis/drug effects; Endotoxins/metabolism; Endotoxins/toxicity; Exocytosis/drug effects; Gene Expression Regulation/drug effects; Hemolysin Proteins/metabolism; Hemolysin Proteins/toxicity; Intracellular Space/metabolism; Manduca/cytology; Manduca/drug effects; Manduca/metabolism; Molecular Sequence Data; Receptors, Cell Surface/genetics; Receptors, Cell Surface/metabolism; Signal Transduction/drug effects
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