PMID:18229619
| Citation |
Zhao, XH, Song, B, Zhong, DF, Zhang, SQ and Chen, XY (2007) [Simultaneous determination of metformin and glipizide in human plasma by liquid chromatography-tandem mass spectrometry]. Yao Xue Xue Bao 42:1087-91 |
|---|---|
| Abstract |
To develop a sensitive and rapid liquid chromatographic-tandem mass spectrometric (LC-MS/MS) method for simultaneous quantitation of metformin and glipizide in human plasma, metformin, glipizide and internal standard diphenhydramine were separated from plasma by protein precipitation with acetonitrile (containing 0.3% formic acid), then chromatographed by using a Zorbax Extend C18 column. The mobile phase consisted of acetonitrile-water-formic acid (70:30: 0.3, v/v/v), at a flow rate of 0.50 mL x min(-1). A tandem mass spectrometer equipped with atmospheric pressure chemical ionization source was used as detector and operated in the positive ion mode. Selected reaction monitoring (SRM) using the precursor/production combinations of m/z 130-->m/z 60, m/z 446-->m/z 321 and m/z 256-->m/z 167 were used to quantify metformin, glipizide and diphenhydramine, respectively. The linear concentration ranges of the calibration curves for metformin and glipizide were 2.00 - 2000 ng x mL(-1) and 1.00 - 1000 ng x mL(-1), respectively. The lower limits of quantitation of metformin and glipizide were 2.00 ng x mL(-1) and 1.00 ng x mL(-1), respectively. The method proved to be sensitive, simple and rapid, and suitable for clinical investigation of compound preparation containing metformin and glipizide. |
| Links | |
| Keywords |
Administration, Oral; Chromatography, Liquid/methods; Glipizide/administration & dosage; Glipizide/blood; Glipizide/pharmacokinetics; Humans; Hypoglycemic Agents/administration & dosage; Hypoglycemic Agents/blood; Hypoglycemic Agents/pharmacokinetics; Male; Metformin/administration & dosage; Metformin/blood; Metformin/pharmacokinetics; Sensitivity and Specificity; Tandem Mass Spectrometry/methods; Young Adult |
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