PMID:18228387

Citation	Pines, J, Jackman, M and Simpson, K (2001) Assays for CDK activity and DNA replication in the cell cycle. Curr Protoc Cell Biol Chapter 8:Unit 8.2
Abstract	This unit describes two assays for different stages of the cell cycle in tissue culture cells. One is a biochemical measurement that assays the protein kinase activity of different cyclin-dependent kinase complexes that are present in late G1 phase, S phase, G2 phase, or mitosis. The other assay uses immunofluorescence to detect DNA replication by the incorporation of nucleotide analogs into DNA. These assays are useful in analyzing the stage and degree of synchrony of the cell cycle and changes in the basic cell cycle machinery.
Links	PubMed Online version:10.1002/0471143030.cb0802s00
Keywords	Animals; Bromodeoxyuridine/analysis; Cell Cycle/physiology; Cells, Cultured/cytology; Cells, Cultured/enzymology; Cyclin-Dependent Kinases/analysis; Cyclin-Dependent Kinases/physiology; Cyclins/physiology; DNA Replication; Immunoprecipitation; Indicators and Reagents; Mammals/anatomy & histology; Phosphorus Radioisotopes/analysis
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