PMID:18228379
| Citation |
Bonifacino, JS (2001) Metabolic labeling with amino acids. Curr Protoc Cell Biol Chapter 7:Unit 7.1 |
|---|---|
| Abstract |
Metabolic labeling techniques are used to study the biosynthesis, processing, intracellular transport, secretion, degradation, and physical-chemical properties of proteins. This unit provides protocols for metabolic labeling of cells with [(35)S]-labeled amino acids to provide material suitable for analysis by immunoprecipitation, for characterization of cellular proteins, for analysis of protein trafficking, and for one- and two-dimensional gel electrophoresis. Three procedures are described for suspension and adherent cells: pulse-labeling, pulse-chase labeling, or continuous long-term labeling. A support protocol describes TCA precipitation to measure the extent of labeling. |
| Links |
PubMed Online version:10.1002/0471143030.cb0701s00 |
| Keywords |
Amino Acids/analysis; Amino Acids/metabolism; Animals; Cells, Cultured/metabolism; Chemical Precipitation; Isotope Labeling/methods; Mammals/metabolism; Methionine/analysis; Methionine/metabolism; Proteins/analysis; Proteins/isolation & purification; Proteins/metabolism; Sulfur Radioisotopes/analysis; Sulfur Radioisotopes/metabolism; Trichloroacetic Acid |
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