PMID:18228310
| Citation |
Yin, YL and Dutta, A (2001) In vitro analysis of SV40 DNA replication. Curr Protoc Cell Biol Chapter 11:Unit 11.5 |
|---|---|
| Abstract |
This unit describes a method for studying cellular factors that affect eukaryotic replication by using a plasmid that contains an SV40 origin of replication and supplying the viral helicase T antigen. Replication factors are supplied by S100 extracts of 293 cells. The unit also contains protocols for expression and purification of T antigen and analysis of the products of replication. |
| Links |
PubMed Online version:10.1002/0471143030.cb1105s02 |
| Keywords |
Antigens, Polyomavirus Transforming/genetics; Cell Line; DNA Replication/physiology; Humans; Kidney/cytology; Molecular Biology/methods; Plasmids/genetics |
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