PMID:18218344
| Citation |
Lee, HS, Kang, IM, Lee, HW, Seo, JH, Ryu, JH, Choi, SJ, Lee, MJ, Jeong, SY, Cho, YW and Lee, KT (2008) Development and validation of a high performance liquid chromatography-tandem mass spectrometry for the determination of etodolac in human plasma. J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. 863:158-62 |
|---|---|
| Abstract |
A simple and specific method using a one-step liquid-liquid extraction (LLE) with butyl acetate followed by high performance liquid chromatography (HPLC) coupled with positive ion electrospray ionization tandem mass spectrometry (ESI-MS/MS) detection was developed for the determination of etodolac in human plasma, using indomethacin as an internal standard (IS). Chromatographic separation was performed isocratically using a Capcellpak MGII C(18) column with 65% acetonitrile and 35% water containing 10mM ammonium formate (adjusted to pH 3.5 with formic acid). Acquisition was performed in multiple reaction monitoring (MRM) mode by monitoring the transitions: m/z 287.99>172.23 for etodolac and m/z 357.92>139.01 for IS. The method was validated to determine its selectivity, linearity, sensitivity, precision, accuracy, recovery and stability. The limit of quantitation (LLOQ) was 0.1microg/mL with a relative standard deviation of less than 15%. The devised method provides an accurate, precise and sensitive tool for determining etodolac levels in plasma. |
| Links |
PubMed Online version:10.1016/j.jchromb.2007.11.032 |
| Keywords |
Anti-Inflammatory Agents, Non-Steroidal/blood; Calibration; Chromatography, High Pressure Liquid; Etodolac/blood; Humans; Indomethacin/blood; Indomethacin/pharmacokinetics; Quality Control; Reproducibility of Results; Tandem Mass Spectrometry |
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