PMID:18217332

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Citation

Li, IT, Pham, E and Truong, K (2007) Current approaches for engineering proteins with diverse biological properties. Adv. Exp. Med. Biol. 620:18-33

Abstract

In the past two decades, protein engineering has advanced significantly with the emergence of new chemical and genetic approaches. Modification and recombination of existing proteins not only produced novel enzymes used commercially and in research laboratories, but furthermore, they revealed the mechanisms of protein function. In this chapter, we will describe the applications and significance of current protein engineering approaches.

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Keywords

Animals; Bacteria/metabolism; Binding Sites; Biotechnology/methods; DNA/chemistry; Fungi/metabolism; Genetic Techniques; Humans; Models, Genetic; Mutagenesis; Oligonucleotides/chemistry; Protein Engineering/methods; Proteins/chemistry; Recombinant Fusion Proteins/chemistry; Recombination, Genetic

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