PMID:18216280
| Citation |
Zeng, X, Barros, MH, Shulman, T and Tzagoloff, A (2008) ATP25, a new nuclear gene of Saccharomyces cerevisiae required for expression and assembly of the Atp9p subunit of mitochondrial ATPase. Mol. Biol. Cell 19:1366-77 |
|---|---|
| Abstract |
We report a new nuclear gene, designated ATP25 (reading frame YMR098C on chromosome XIII), required for expression of Atp9p (subunit 9) of the Saccharomyces cerevisiae mitochondrial proton translocating ATPase. Mutations in ATP25 elicit a deficit of ATP9 mRNA and of its translation product, thereby preventing assembly of functional F(0). Unlike Atp9p, the other mitochondrial gene products, including ATPase subunits Atp6p and Atp8p, are synthesized normally in atp25 mutants. Northern analysis of mitochondrial RNAs in an atp25 temperature-sensitive mutant confirmed that Atp25p is required for stability of the ATP9 mRNA. Atp25p is a mitochondrial inner membrane protein with a predicted mass of 70 kDa. The primary translation product of ATP25 is cleaved in vivo after residue 292 to yield a 35-kDa C-terminal polypeptide. The C-terminal half of Atp25p is sufficient to stabilize the ATP9 mRNA and restore synthesis of Atp9p. Growth on respiratory substrates, however, depends on both halves of Atp25p, indicating that the N-terminal half has another function, which we propose to be oligomerization of Atp9p into a proper size ring structure. |
| Links |
PubMed PMC2291438 Online version:10.1091/mbc.E07-08-0746 |
| Keywords |
Alleles; Base Sequence; Cell Nucleus/genetics; Cell Nucleus/metabolism; Cloning, Molecular; DNA, Fungal/genetics; Gene Expression; Genes, Fungal; Genetic Complementation Test; Mitochondrial Proton-Translocating ATPases/chemistry; Mitochondrial Proton-Translocating ATPases/genetics; Mitochondrial Proton-Translocating ATPases/metabolism; Molecular Weight; Mutation; Protein Subunits; RNA, Fungal/genetics; RNA, Fungal/metabolism; RNA, Messenger/genetics; RNA, Messenger/metabolism; Recombinant Proteins/chemistry; Recombinant Proteins/genetics; Recombinant Proteins/metabolism; Saccharomyces cerevisiae/genetics; Saccharomyces cerevisiae/growth & development; Saccharomyces cerevisiae/metabolism; Saccharomyces cerevisiae Proteins/chemistry; Saccharomyces cerevisiae Proteins/genetics; Saccharomyces cerevisiae Proteins/metabolism; Temperature |
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