PMID:18213377

Citation	Charvin, G, Cross, FR and Siggia, ED (2008) A microfluidic device for temporally controlled gene expression and long-term fluorescent imaging in unperturbed dividing yeast cells. PLoS ONE 3:e1468
Abstract	Imaging single cells with fluorescent markers over multiple cell cycles is a powerful tool for unraveling the mechanism and dynamics of the cell cycle. Over the past ten years, microfluidic techniques in cell biology have emerged that allow for good control of growth environment. Yet the control and quantification of transient gene expression in unperturbed dividing cells has received less attention.
Links	PubMed PMC2194624 Online version:10.1371/journal.pone.0001468
Keywords	Cell Cycle; Cell Division; F-Box Proteins; Gene Expression Regulation; Microfluidics/instrumentation; Microscopy, Fluorescence; Promoter Regions, Genetic; Repressor Proteins/genetics; Saccharomyces cerevisiae/cytology; Saccharomyces cerevisiae/genetics; Saccharomyces cerevisiae Proteins/genetics; Transcriptional Activation; Ubiquitin-Protein Ligase Complexes/genetics
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