PMID:18205125
| Citation |
Terrazas, M, Marlière, P and Herdewijn, P (2008) Enzymatically catalyzed DNA synthesis using L-Asp-dGMP, L-Asp-dCMP, and L-Asp-dTMP. Chem. Biodivers. 5:31-9 |
|---|---|
| Abstract |
The replacement of the pyrophosphate moiety of deoxynucleoside triphosphates by L-aspartic acid allows incorporation of natural deoxynucleosides into DNA using HIV reverse transcriptase (RT) as enzyme, while retaining the canonical base-pair selectivity. N-Methylation of the L-aspartic acid leaving group results in a reduced fidelity of incorporation. |
| Links |
PubMed Online version:10.1002/cbdv.200890013 |
| Keywords |
Aspartic Acid/chemistry; Catalysis; DNA/chemical synthesis; DNA/chemistry; Deoxycytidine Monophosphate/chemistry; Deoxyguanine Nucleotides/chemistry; HIV Reverse Transcriptase/chemistry; Methylation; Molecular Conformation; Molecular Structure; Stereoisomerism; Thymidine Monophosphate/chemistry |
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