PMID:18201202
| Citation |
Aoki, H, Xu, J, Emili, A, Chosay, JG, Golshani, A and Ganoza, MC (2008) Interactions of elongation factor EF-P with the Escherichia coli ribosome. FEBS J. 275:671-81 |
|---|---|
| Abstract |
EF-P (eubacterial elongation factor P) is a highly conserved protein essential for protein synthesis. We report that EF-P protects 16S rRNA near the G526 streptomycin and the S12 and mRNA binding sites (30S T-site). EF-P also protects domain V of the 23S rRNA proximal to the A-site (50S T-site) and more strongly the A-site of 70S ribosomes. We suggest that EF-P: (a) may play a role in translational fidelity and (b) prevents entry of fMet-tRNA into the A-site enabling it to bind to the 50S P-site. We also report that EF-P promotes a ribosome-dependent accommodation of fMet-tRNA into the 70S P-site. |
| Links |
PubMed Online version:10.1111/j.1742-4658.2007.06228.x |
| Keywords |
Binding Sites; Escherichia coli/metabolism; Immunoprecipitation; Mass Spectrometry; Peptide Elongation Factors/metabolism; Protein Binding; RNA, Ribosomal, 16S/metabolism; RNA, Ribosomal, 23S/metabolism; Ribosomes/metabolism |
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