PMID:18178732
| Citation |
Morris, P, Marinelli, LJ, Jacobs-Sera, D, Hendrix, RW and Hatfull, GF (2008) Genomic characterization of mycobacteriophage Giles: evidence for phage acquisition of host DNA by illegitimate recombination. J. Bacteriol. 190:2172-82 |
|---|---|
| Abstract |
A characteristic feature of bacteriophage genomes is that they are architecturally mosaic, with each individual genome representing a unique assemblage of individual exchangeable modules. Plausible mechanisms for generating mosaicism include homologous recombination at shared boundary sequences of module junctions, illegitimate recombination in a non-sequence-directed process, and site-specific recombination. Analysis of the novel mycobacteriophage Giles genome not only extends our current perspective on bacteriophage genetic diversity, with more than 60% of the genes unrelated to other mycobacteriophages, but offers novel insights into how mosaic genomes are created. In one example, the integration/excision cassette is atypically situated within the structural gene operon and could have moved there either by illegitimate recombination or more plausibly via integrase-mediated site-specific recombination. In a second example, a DNA segment has been recently acquired from the host bacterial chromosome by illegitimate recombination, providing further evidence that phage genomic mosaicism is generated by nontargeted recombination processes. |
| Links |
PubMed PMC2258872 Online version:10.1128/JB.01657-07 |
| Keywords |
Base Sequence; DNA, Bacterial/genetics; Genome, Viral/genetics; Host-Pathogen Interactions; Microscopy, Electron; Molecular Sequence Data; Mycobacteriophages/genetics; Mycobacteriophages/physiology; Mycobacteriophages/ultrastructure; Mycobacterium smegmatis/genetics; Mycobacterium smegmatis/virology; Operon/genetics; Recombination, Genetic; Sequence Homology, Nucleic Acid |
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