PMID:18175933

Citation	Ohara, T, Ohashi-Kobayashi, A and Maeda, M (2008) Biochemical characterization of transporter associated with antigen processing (TAP)-like (ABCB9) expressed in insect cells. Biol. Pharm. Bull. 31:1-5
Abstract	The ATP-binding cassette (ABC) transporter, transporter associated with antigen processing (TAP)-like (TAPL) tagged with a histidine cluster was overexpressed, amounting to as much as 1-2% of total membrane proteins in Drosophila cell line S2. TAPL was effectively solubilized from membranes by Triton X-100, NP-40 and n-dodecyl-beta-D-maltoside. Solubilized TAPL bound ATP-agarose and adenosine 5'-diphosphate (ADP)-agarose but not adenosine 5'-monophosphate (AMP)-agarose. The binding was competed for by excess free ATP, ADP, guanosine 5'-triphosphate (GTP) and dATP but not by AMP. Pyrimidine nucleotides such as uridine 5'-triphosphate (UTP) and cytidine 5'-triphosphate (CTP) were less effective competitors, suggesting that purine nucleotide triphosphates are substrates for TAPL. The ATP-binding of TAPL required Mg(2+), and was observed at neutral pH. Chemical cross-linking experiments suggested that TAPL forms a homodimer in the membrane and under the solubilized conditions.
Links	PubMed
Keywords	ATP-Binding Cassette Transporters/chemistry; ATP-Binding Cassette Transporters/metabolism; Adenosine Diphosphate/metabolism; Adenosine Triphosphate/metabolism; Animals; Cell Line; Dimerization; Drosophila; Recombinant Proteins/metabolism; Solubility
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