PMID:18174133
| Citation |
Kurth, EG, Doughty, DM, Bottomley, PJ, Arp, DJ and Sayavedra-Soto, LA (2008) Involvement of BmoR and BmoG in n-alkane metabolism in 'Pseudomonas butanovora'. Microbiology (Reading, Engl.) 154:139-47 |
|---|---|
| Abstract |
'Pseudomonas butanovora' uses an alcohol-inducible alkane monooxygenase (BMO) to grow on C(2)-C(9) n-alkanes. Five ORFs were identified flanking the BMO structural genes. Two of the ORFs, bmoR, encoding a putative sigma(54)-transcriptional regulator BmoR, and bmoG, encoding a putative GroEL chaperonin BmoG, were analysed by gene-inactivation experiments. The BmoR-deficient mutant grew at slower growth rates than the wild-type on C(2)-C(5) n-alkanes and showed little to no growth on C(6)-C(8) n-alkanes within 7 days. A BmoR-deficient mutant was constructed in the 'P. butanovora' bmoX : : lacZ reporter strain and used to test whether bmoR was involved in bmoX induction after growth on C(2)-C(8) carbon sources. In acetate- or lactate-grown cells, C(2)-C(8) n-alcohols failed to induce beta-galactosidase activity. In contrast, in propionate-, butyrate- or pentanoate-grown cells, n-butanol induced approximately 45 % of the beta-galactosidase activity observed in the control bmoX : : lacZ strain. In propionate-grown cells, C(2)-C(5) n-alcohols induced beta-galactosidase activity, whereas C(7) and C(8) n-alcohols did not. BmoR may act as a sigma(54)-transcriptional regulator of bmo that is controlled by the n-alcohol produced in the alkane oxidation. During growth on short-chain-length fatty acids, however, another BMO regulatory system seems to be activated to promote transcription of bmo by short-chain-length alcohols (i.e. </=C(6)). The bmoG-deficient mutant did not grow on C(2)-C(8) n-alkanes; however, it was capable of transcribing bmoX and bmoC of the BMO operon. BmoG may act as a chaperonin to assemble competent BMO. |
| Links |
PubMed Online version:10.1099/mic.0.2007/012724-0 |
| Keywords |
Acetates/metabolism; Alcohols/metabolism; Alkanes/metabolism; Butyrates/metabolism; Chaperonins/genetics; Chaperonins/metabolism; DNA, Bacterial/chemistry; DNA, Bacterial/genetics; Fatty Acids, Volatile/metabolism; Gene Deletion; Gene Expression Regulation; Genes, Reporter; Lactates/metabolism; Models, Biological; Molecular Chaperones/genetics; Molecular Chaperones/metabolism; Molecular Sequence Data; Mutagenesis, Insertional; Propionates/metabolism; Pseudomonas/genetics; Pseudomonas/growth & development; Pseudomonas/metabolism; Sequence Analysis, DNA; Sigma Factor/genetics; Sigma Factor/metabolism; Transcription Factors/genetics; Transcription Factors/metabolism; beta-Galactosidase/analysis; beta-Galactosidase/genetics |
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