PMID:18164874
| Citation |
Rossi, M, Hänninen, ML, Revez, J, Hannula, M and Zanoni, RG (2008) Occurrence and species level diagnostics of Campylobacter spp., enteric Helicobacter spp. and Anaerobiospirillum spp. in healthy and diarrheic dogs and cats. Vet. Microbiol. 129:304-14 |
|---|---|
| Abstract |
In order to study the occurrence and co-infection of different species of Campylobacter, enteric Helicobacter and Anaerobiospirillum in dogs and cats and define a possible association between these microrganisms and gastrointestinal disorders, 190 dogs and 84 cats, either healthy or with diarrhea, were sampled between 2002 and 2003. Thirty-three C. upsaliensis, 17 C. jejuni, 2 C. helveticus, 1 C. lari isolates from dogs and 14 C. helveticus, 7 C. jejuni, 6 C. upsaliensis isolates from cats were identified using species-specific PCR and phenotypic tests. Whole cell protein profile analysis, phenotypic tests, PCR-RFLP of gyrB and a phylogenetic study of partial groEL and 16S rRNA sequences were used to identify 37 H. bilis, 22 H. canis and 14 H. cinaedi in dogs and 12 H. canis, 5 H. bilis and 2 H. cinaedi in cats. Whole cell protein profile analysis, phenotypic tests and species-specific PCR of 16S rRNA were used to identify 14 A. succiniciproducens, 12 A. thomasii isolates and one unidentified Anaerobiospirillum sp. isolate in dogs and 3 A. thomasii isolates in cats. Fifty-two animals (19%) were positive for the isolation of more than one genus. No significant statistical correlation was found between any isolates of Campylobacter, Helicobacter or Anaerobiospirillum spp. or the various co-infection rates, and the presence of diarrhea in either dogs or cats. Campylobacter isolates were also tested for antibiotic resistance using the agar dilution method. |
| Links |
PubMed Online version:10.1016/j.vetmic.2007.11.014 |
| Keywords |
Anaerobiospirillum/classification; Anaerobiospirillum/drug effects; Anaerobiospirillum/isolation & purification; Animals; Anti-Bacterial Agents/therapeutic use; Campylobacter/classification; Campylobacter/drug effects; Campylobacter/isolation & purification; Campylobacter Infections/diagnosis; Campylobacter Infections/drug therapy; Campylobacter Infections/microbiology; Campylobacter Infections/veterinary; Cat Diseases/diagnosis; Cat Diseases/drug therapy; Cat Diseases/microbiology; Cats/microbiology; Diarrhea/diagnosis; Diarrhea/drug therapy; Diarrhea/microbiology; Diarrhea/veterinary; Dog Diseases/diagnosis; Dog Diseases/drug therapy; Dog Diseases/microbiology; Dogs/microbiology; Dose-Response Relationship, Drug; Drug Resistance, Bacterial; Gram-Negative Bacterial Infections/diagnosis; Gram-Negative Bacterial Infections/drug therapy; Gram-Negative Bacterial Infections/microbiology; Gram-Negative Bacterial Infections/veterinary; Helicobacter/classification; Helicobacter/drug effects; Helicobacter/isolation & purification; Helicobacter Infections/diagnosis; Helicobacter Infections/drug therapy; Helicobacter Infections/microbiology; Helicobacter Infections/veterinary; Microbial Sensitivity Tests/veterinary; Molecular Sequence Data; Phylogeny; Polymerase Chain Reaction/methods; Polymerase Chain Reaction/veterinary; Prevalence; RNA, Ribosomal, 16S/genetics; Species Specificity |
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