PMID:18164681
| Citation |
Chang, LJ, Chen, WH, Minion, FC and Shiuan, D (2008) Mycoplasmas regulate the expression of heat-shock protein genes through CIRCE-HrcA interactions. Biochem. Biophys. Res. Commun. 367:213-8 |
|---|---|
| Abstract |
Mycoplasmas in general are rarely exposed to severe environmental changes except during its colonization and infection processes. Genomic analysis indicates that Mycoplasma hyopneumoniae possesses the genes of a single sigma factor and the HrcA repressor of negative regulation of the heat-shock response. A perfect inverted repeat sequence (5'-CTGGCACTT-N(9)-AAGTGCCAA-3') upstream of the DnaK gene has also been identified. In the present study, we demonstrate the functionality of HrcA-CIRCE interactions using the gel electrophoretic mobility shift assay. The presence of the unique sigma factor, HrcA repressor, and the CIRCE-like sequences reveals that mycoplasmal species may all use the negative regulatory mechanism in the heat-shock response. It is conceivable that mycoplasmas may have evolved a single HrcA repressor-based mechanism which might be the most simple and economical way of controlling HSP gene expression. |
| Links |
PubMed Online version:10.1016/j.bbrc.2007.12.124 |
| Keywords |
Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Base Sequence; DNA-Binding Proteins/genetics; DNA-Binding Proteins/metabolism; Electrophoretic Mobility Shift Assay; Escherichia coli Proteins/genetics; Escherichia coli Proteins/metabolism; Gene Expression Regulation, Bacterial; Genes, Regulator; Genomics; HSP70 Heat-Shock Proteins/genetics; HSP70 Heat-Shock Proteins/metabolism; Heat-Shock Proteins/genetics; Heat-Shock Proteins/metabolism; Heat-Shock Response/genetics; Heat-Shock Response/physiology; Molecular Sequence Data; Mycoplasma hyopneumoniae/genetics; Mycoplasma hyopneumoniae/physiology; Regulatory Sequences, Nucleic Acid/genetics; Regulatory Sequences, Nucleic Acid/physiology; Repressor Proteins/genetics; Repressor Proteins/metabolism; Sigma Factor/genetics; Sigma Factor/metabolism |
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