PMID:18157151
| Citation |
Costantino, DA, Pfingsten, JS, Rambo, RP and Kieft, JS (2008) tRNA-mRNA mimicry drives translation initiation from a viral IRES. Nat. Struct. Mol. Biol. 15:57-64 |
|---|---|
| Abstract |
Internal ribosome entry site (IRES) RNAs initiate protein synthesis in eukaryotic cells by a noncanonical cap-independent mechanism. IRESes are critical for many pathogenic viruses, but efforts to understand their function are complicated by the diversity of IRES sequences as well as by limited high-resolution structural information. The intergenic region (IGR) IRESes of the Dicistroviridae viruses are powerful model systems to begin to understand IRES function. Here we present the crystal structure of a Dicistroviridae IGR IRES domain that interacts with the ribosome's decoding groove. We find that this RNA domain precisely mimics the transfer RNA anticodon-messenger RNA codon interaction, and its modeled orientation on the ribosome helps explain translocation without peptide bond formation. When combined with a previous structure, this work completes the first high-resolution description of an IRES RNA and provides insight into how RNAs can manipulate complex biological machines. |
| Links |
PubMed PMC2748805 Online version:10.1038/nsmb1351 |
| Keywords |
Anticodon/genetics; Binding Sites; Hepacivirus/genetics; Models, Genetic; Models, Molecular; Nucleic Acid Conformation; Protein Biosynthesis; RNA, Messenger/chemistry; RNA, Messenger/genetics; RNA, Messenger/metabolism; RNA, Transfer/chemistry; RNA, Transfer/genetics; RNA, Transfer/metabolism; RNA, Viral/chemistry; RNA, Viral/genetics; RNA, Viral/metabolism; Ribosomes/genetics; Ribosomes/physiology |
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