PMID:18093330

Citation	Mickle, KL, Ramanathan, S, Rosebrock, A, Oliva, A, Chaudari, A, Yompakdee, C, Scott, D, Leatherwood, J and Huberman, JA (2007) Checkpoint independence of most DNA replication origins in fission yeast. BMC Mol. Biol. 8:112
Abstract	In budding yeast, the replication checkpoint slows progress through S phase by inhibiting replication origin firing. In mammals, the replication checkpoint inhibits both origin firing and replication fork movement. To find out which strategy is employed in the fission yeast, Schizosaccharomyces pombe, we used microarrays to investigate the use of origins by wild-type and checkpoint-mutant strains in the presence of hydroxyurea (HU), which limits the pool of deoxyribonucleoside triphosphates (dNTPs) and activates the replication checkpoint. The checkpoint-mutant cells carried deletions either of rad3 (which encodes the fission yeast homologue of ATR) or cds1 (which encodes the fission yeast homologue of Chk2).
Links	PubMed PMC2235891 Online version:10.1186/1471-2199-8-112
Keywords	Cell Cycle Proteins/genetics; Chromosomes/genetics; DNA Replication/drug effects; DNA Replication/physiology; DNA, Fungal/genetics; DNA, Fungal/physiology; Hydroxyurea/pharmacology; Microarray Analysis/methods; Mutation; Nucleic Acid Synthesis Inhibitors/pharmacology; Protein Kinases/genetics; Protein-Serine-Threonine Kinases/genetics; Replication Origin/genetics; Schizosaccharomyces/genetics; Schizosaccharomyces pombe Proteins/genetics
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