PMID:18071247
| Citation |
Gullapalli, P, Takata, G, Poonperm, W, Rao, D, Morimoto, K, Akimitsu, K, Tajima, S and Izumori, K (2007) Bioproduction of D-psicose from allitol with Enterobacter aerogenes IK7: a new frontier in rare ketose production. Biosci. Biotechnol. Biochem. 71:3048-54 |
|---|---|
| Abstract |
D-psicose, a new alternative sweetener, was produced from allitol by microbial oxidation of the newly isolated strain Enterobacter aerogenes IK7. Cells grown in tryptic soy broth medium (TSB) supplemented with D-mannitol at 37 degrees C were found to have the best oxidation potential. The cells, owing to broad substrate specificity, oxidized various polyols (tetritol, pentitol, and hexitol) to corresponding rare ketoses. By a resting cell reaction, 10% of allitol was completely transformed to the product D-psicose, which thus becomes economically feasible for the mass production of D-psicose. Finally, the product was crystallized and confirmed to be D-psicose by analytical methods. |
| Links | |
| Keywords |
Enterobacter aerogenes/metabolism; Fructose/chemistry; Fructose/metabolism; Oxidation-Reduction; Stereoisomerism; Sugar Alcohols/metabolism; Sweetening Agents/chemistry; Sweetening Agents/metabolism |
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