PMID:18065458
| Citation |
Edwards, MD, Bartlett, W and Booth, IR (2008) Pore mutations of the Escherichia coli MscS channel affect desensitization but not ionic preference. Biophys. J. 94:3003-13 |
|---|---|
| Abstract |
Mechanosensitive channels rescue bacterial cells from a fate of lysis when they transfer from a high- to low-osmolarity environment. Of three Escherichia coli mechanosensitive proteins studied to date, only MscS-Ec demonstrates a small anionic preference and a desensitized, nonconducting state under sustained pressure. Little is known about the mechanisms generating these distinctive properties. Eliminating the sole positive charge in the MscS-Ec pore region (Arg(88)) did not alter anionic preference. Adding positive charges at either end of the pore did not augment anionic preference, and placing negative charges within the pore did not diminish it. Thus, pore charges do not control this characteristic. However, from this analysis we identified mutations in the hinge region of the MscS-Ec pore helix (at Gly(113)) that profoundly affected ability of the channel to desensitize. Substitution with nonpolar (Ala, Pro) or polar (Asp, Arg, Ser) residues inhibited transition to the desensitized state. Interestingly, Gly(113) replaced with Met did not impede desensitization. Thus, although Gly is not specifically required at position 113, MscS desensitization is strongly influenced by the residue situated here. Mutations at residues further into the pore also regulated desensitization. Transition to this unique mechanosensitive channel state is discussed in terms of existing data. |
| Links |
PubMed PMC2275705 Online version:10.1529/biophysj.107.123448 |
| Keywords |
Amino Acid Substitution; Cell Membrane/chemistry; Cell Membrane/physiology; Computer Simulation; Escherichia coli Proteins/chemistry; Escherichia coli Proteins/genetics; Ion Channel Gating/physiology; Ion Channels/chemistry; Ion Channels/genetics; Lipid Bilayers/chemistry; Mechanotransduction, Cellular/physiology; Models, Biological; Models, Chemical; Models, Molecular; Mutagenesis, Site-Directed; Porosity; Stress, Mechanical |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
See also
References
See Help:References for how to manage references in EcoliWiki.