PMID:1805966
| Citation |
Mikuni, O, Kawakami, K and Nakamura, Y (1991) Sequence and functional analysis of mutations in the gene encoding peptide-chain-release factor 2 of Escherichia coli. Biochimie 73:1509-16 |
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| Abstract |
Mutations in the prfB gene which encodes peptide-chain-release factor 2 of Escherichia coli were defined by DNA sequence analysis. prfB1 and prfB3 substitute lysine and asparagine for glutamate and aspartate at amino acid positions 89 and 143, respectively. Temperature-sensitive mutations, prfB2 and prfB286, each contain the identical substitution of phenylalanine for leucine-328. These mutations suppress UGA but not UAG or UAA. The efficiency of suppression was affected by the neighboring RNA context. The prfB gene encodes a premature UGA stop codon at position 26 and is expressed by +1 frameshifting. The efficiency of natural frameshift was 18% as measured by using the monolysogenic lambda assay vector containing prfB-lacZ fusions, and increased up to 30% in the prfB mutants. These observations can be interpreted as genetic evidence for the autogenous control of RF2 synthesis by frameshifting. Structural and functional organizations of release factors are discussed. |
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| Keywords |
Amino Acid Sequence; Asparagine/chemistry; Blotting, Southern; Codon; Escherichia coli/genetics; Lysine/chemistry; Molecular Sequence Data; Mutation; Peptide Chain Termination, Translational; Peptide Termination Factors/biosynthesis; Peptide Termination Factors/genetics; Protein Biosynthesis; Suppression, Genetic |
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