PMID:18048691
| Citation |
Jeoung, JH and Dobbek, H (2007) Carbon dioxide activation at the Ni,Fe-cluster of anaerobic carbon monoxide dehydrogenase. Science 318:1461-4 |
|---|---|
| Abstract |
Anaerobic CO dehydrogenases catalyze the reversible oxidation of CO to CO2 at a complex Ni-, Fe-, and S-containing metal center called cluster C. We report crystal structures of CO dehydrogenase II from Carboxydothermus hydrogenoformans in three different states. In a reduced state, exogenous CO2 supplied in solution is bound and reductively activated by cluster C. In the intermediate structure, CO2 acts as a bridging ligand between Ni and the asymmetrically coordinated Fe, where it completes the square-planar coordination of the Ni ion. It replaces a water/hydroxo ligand bound to the Fe ion in the other two states. The structures define the mechanism of CO oxidation and CO2 reduction at the Ni-Fe site of cluster C. |
| Links |
PubMed Online version:10.1126/science.1148481 |
| Keywords |
Aldehyde Oxidoreductases/chemistry; Aldehyde Oxidoreductases/isolation & purification; Aldehyde Oxidoreductases/metabolism; Anaerobiosis; Binding Sites; Carbon Dioxide/metabolism; Carbon Monoxide/metabolism; Crystallization; Crystallography, X-Ray; Iron/chemistry; Iron/metabolism; Ligands; Multienzyme Complexes/chemistry; Multienzyme Complexes/isolation & purification; Multienzyme Complexes/metabolism; Nickel/chemistry; Nickel/metabolism; Peptococcaceae/enzymology; Protein Conformation; Recombinant Proteins/chemistry; Recombinant Proteins/isolation & purification; Recombinant Proteins/metabolism |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.