PMID:18029734
| Citation |
Imoto, S, Ali, MM, Li, Y, Sasaki, S and Nagatsugi, F' (2007) Enzymatic incorporation of 2-amino-6-vinylpurine derivative. Nucleic Acids Symp Ser (Oxf) ':357-8 |
|---|---|
| Abstract |
Modified nucleotides are incorporated by enzymatic reactions into a DNA oligonulceotide to provide additional functionalities, which has shown potential applications in a wide variety of fields. A large number of modified nucleotide triphosphates have been incorporated to DNA or RNA by polymerases. We have previously described that the sulfide derivatives (1) of 2-amino-6-vinyl purine nucleoside (2, AVP) are activated within duplex to form cross-link toward cytidine selectively at the target site. In this paper, we describe the preparation of the triphosphate derivative of 2'-deoxyribosyl-2-amino-6-(2-methylthioethyl) purine nucleoside (5, dAVP(S Me)-TP), and its enzymatic incorporation to DNA. |
| Links |
PubMed Online version:10.1093/nass/nrm179 |
| Keywords |
DNA/biosynthesis; DNA/chemistry; DNA-Directed DNA Polymerase/metabolism; Purine Nucleotides/chemical synthesis; Purine Nucleotides/chemistry; Purine Nucleotides/metabolism |
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