PMID:18024517

Citation	Gourion, B, Francez-Charlot, A and Vorholt, JA (2008) PhyR is involved in the general stress response of Methylobacterium extorquens AM1. J. Bacteriol. 190:1027-35
Abstract	PhyR represents a novel alphaproteobacterial family of response regulators having a structure consisting of two domains; a predicted amino-terminal extracytoplasmic function (ECF) sigma factor-like domain and a carboxy-terminal receiver domain. PhyR was first described in Methylobacterium extorquens AM1, in which it has been shown to be essential for plant colonization, probably due to its suggested involvement in the regulation of a number of stress proteins. Here we investigated the PhyR regulon using microarray technology. We found that the PhyR regulon is rather large and that most of the 246 targets are under positive control. Mapping of transcriptional start sites revealed candidate promoters for PhyR-mediated regulation. One of these promoters, an ECF-type promoter, was identified upstream of one-third of the target genes by in silico analysis. Among the PhyR targets are genes predicted to be involved in multiple stress responses, including katE, osmC, htrA, dnaK, gloA, dps, and uvrA. The induction of these genes is consistent with our phenotypic analyses which revealed that PhyR is involved in resistance to heat shock and desiccation, as well as oxidative, UV, ethanol, and osmotic stresses, in M. extorquens AM1. The finding that PhyR is involved in the general stress response was further substantiated by the finding that carbon starvation induces protection against heat shock and that this protection is at least in part dependent on PhyR.
Links	PubMed PMC2223570 Online version:10.1128/JB.01483-07
Keywords	Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Base Sequence; Gene Expression Profiling; Gene Expression Regulation, Bacterial; Genes, Regulator; Heat-Shock Response; Methylobacterium extorquens/genetics; Methylobacterium extorquens/metabolism; Methylobacterium extorquens/physiology; Molecular Sequence Data; Oligonucleotide Array Sequence Analysis; Proteome; Sequence Analysis, DNA
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