PMID:18007030

Citation	Förster, C, Brauer, AB, Brode, S, Fürste, JP, Betzel, C and Erdmann, VA (2007) tRNASer acceptor stem: conformation and hydration of a microhelix in a crystal structure at 1.8 A resolution. Acta Crystallogr. D Biol. Crystallogr. 63:1154-61
Abstract	The crystal structure of a serine-specific tRNA acceptor-stem microhelix, the binding site for the seryl-tRNA synthetase, was solved by X-ray analysis. This seven-base-pair tRNA(Ser) microhelix forms endless rows of helices in the crystal lattice, with two helices stacking 'head-to-head' onto each other, resulting in an intermolecular guanosine stacking of the first purine nucleotides at the 5'-strands of the tRNA(Ser) microhelices. A network of 75 water loci could be associated with each RNA duplex. Unusual local geometric backbone parameters could be detected in the region of the G4 phosphate located in the 5'-strand of the helix, which lead to a ;kink' in this region and to an irregularly bent helix. The role of the specific hydration pattern and of the irregular conformation of the tRNA(Ser) acceptor-stem helix is discussed and summarized.
Links	PubMed Online version:10.1107/S0907444907045271
Keywords	Base Sequence; Crystallography, X-Ray; Escherichia coli/chemistry; Hydrogen Bonding; Models, Molecular; Nucleic Acid Conformation; RNA, Transfer, Ser/chemistry; Water/chemistry
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