PMID:17964284
| Citation |
Jiang, PX, Feng, Y and He, ZG (2007) Functional differentiation and cooperative interaction between two eukaryote-like archaeal Orc1/Cdc6 proteins on the replication origin. Biochem. Biophys. Res. Commun. 364:945-51 |
|---|---|
| Abstract |
The DNA replication apparatus of archaea represents a core version of that in eukaryotes. Archaeal Orc1/Cdc6s can be an integral component in the replication machineries cooperatively regulating DNA replication. We investigated the DNA-binding activities of two eukaryote-like Orc1/Cdc6 proteins (SsoCdc6-1 and -2) and interactions between them on the different structural duplex DNA substrates derived from oriC1 of Sulfolobus solfataricus. The results showed that two Orc1/Cdc6 proteins stimulated mutual DNA-binding activities at lower concentrations and formed bigger SsoCdc6-1/SsoCdc6-2/DNA complex at higher concentrations. Furthermore, SsoCdc6-2 stimulated the DNA-binding activity of SsoMCM and demonstrated a high affinity to the 5-forked DNA. In contrast, SsoCdc6-1 inhibited the binding of SsoMCM and demonstrated better affinity to the sequence-specific blunt DNA substrate. Finally, we found that the two proteins physically interacted with each other and with SsoMCM. Thus, the two Orc1/Cdc6 proteins were functionally different, but they may keep the coordinated interaction on the replication origin. |
| Links |
PubMed Online version:10.1016/j.bbrc.2007.10.099 |
| Keywords |
Cell Cycle Proteins/metabolism; DNA Replication/physiology; Origin Recognition Complex/metabolism; Replication Origin/physiology; Saccharomyces cerevisiae Proteins/metabolism; Sulfolobus/metabolism |
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