PMID:17917748
| Citation |
Li, M and Wang, B (2007) Homology modeling and examination of the effect of the D92E mutation on the H5N1 nonstructural protein NS1 effector domain. J Mol Model 13:1237-44 |
|---|---|
| Abstract |
Virulent H5N1 strains of influenza virus often harbor a D92E point mutation in the nonstructural protein NS1. This crucial mutation has been correlated with increased virulence and/or cytokine resistance, but the structural implications of such a change are still unclear. Furthermore, NS1 protein could also be a potential target for the development of novel antiviral agents against H5N1 strains. Therefore, a reasonable 3D model of H5N1 NS1 is important for the understanding of the molecular basis of increased virulence and the design of novel antiviral agents. Based on the crystal structure of a non-H5N1 NS1 protein, a model of H5N1 NS1 was developed by homology modeling, molecular mechanics and molecular dynamics simulations. It was found that the D92E mutation could result in weakened interactions of the carboxylate side chain with other phosphorylated residues, thereby activating phosphorylation of NS1. |
| Links |
PubMed Online version:10.1007/s00894-007-0245-0 |
| Keywords |
Amino Acid Sequence; Aspartic Acid/genetics; Aspartic Acid/metabolism; Computer Simulation; Glutamic Acid/genetics; Glutamic Acid/metabolism; Influenza A Virus, H5N1 Subtype/chemistry; Influenza A Virus, H5N1 Subtype/genetics; Influenza A Virus, H5N1 Subtype/metabolism; Models, Molecular; Molecular Sequence Data; Mutation/genetics; Phosphorylation; Protein Kinases/metabolism; Protein Structure, Tertiary; Sequence Alignment; Sequence Homology, Amino Acid; Structural Homology, Protein; Viral Nonstructural Proteins/chemistry; Viral Nonstructural Proteins/genetics; Viral Nonstructural Proteins/metabolism |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.