PMID:1791763
| Citation |
Bihimaier, A, Römling, U, Meyer, TF, Tümmler, B and Gibbs, CP (1991) Physical and genetic map of the Neisseria gonorrhoeae strain MS11-N198 chromosome. Mol. Microbiol. 5:2529-39 |
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| Abstract |
A macro-restriction map of the Neisseria gonorrhoeae chromosome was constructed using the enzymes Nhel and Spel. Combinations of one- and two-dimensional electrophoresis of completely or partially digested chromosomal DNA were performed to align the restriction fragments. The chromosome is circular, with an estimated size of 2.33 Mb +/- 35 kb. A genetic map was derived from the physical map; positions of over 60 defined loci were determined by Southern hybridization. |
| Links | |
| Keywords |
Blotting, Southern; Chromosome Mapping; Chromosomes, Bacterial; DNA, Bacterial/genetics; DNA, Bacterial/isolation & purification; Electrophoresis, Gel, Two-Dimensional; Electrophoresis, Polyacrylamide Gel; Neisseria gonorrhoeae/genetics; Plasmids; Restriction Mapping |
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